PubMed:9119228 JSONTXT

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{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":100},"obj":"Sentence"},{"id":"S2","span":{"begin":101,"end":311},"obj":"Sentence"},{"id":"S3","span":{"begin":312,"end":539},"obj":"Sentence"},{"id":"S4","span":{"begin":540,"end":680},"obj":"Sentence"},{"id":"S5","span":{"begin":681,"end":904},"obj":"Sentence"},{"id":"S6","span":{"begin":905,"end":978},"obj":"Sentence"},{"id":"S7","span":{"begin":979,"end":1234},"obj":"Sentence"},{"id":"S8","span":{"begin":1235,"end":1409},"obj":"Sentence"},{"id":"S9","span":{"begin":1410,"end":1584},"obj":"Sentence"}],"text":"ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCRalpha enhancer function.\nLEF-1 is a transcription factor that participates in the regulation of the T-cell receptor alpha (TCR alpha) enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. The function of LEF-1 is dependent, in part, on the HMG domain that induces a sharp bend in the DNA helix, and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins. With the aim of gaining insight into the function of context-dependent activation domains, we cloned ALY, a novel LEF-1-interacting protein. ALY is a ubiquitously expressed, nuclear protein that specifically associates with the activation domains of LEF-1 and AML-1 (CBF alpha2, PEBP2 alpha(B), which is another protein component of the TCR alpha enhancer complex. In addition, ALY can increase DNA binding by both LEF-1 and AML proteins. Overexpression of ALY stimulates the activity of the TCR alpha enhancer complex reconstituted in transfected nonlymphoid HeLa cells, whereas down-regulation of ALY by anti-sense oligonucleotides virtually eliminates TCR alpha enhancer activity in T cells. Similar to LEF-1, ALY can stimulate transcription in the context of the TCR alpha enhancer but apparently not when tethered to DNA through an heterologous DNA-binding domain. We propose that ALY mediates context-dependent transcriptional activation by facilitating the functional collaboration of multiple proteins in the TCR alpha enhancer complex."}

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