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PubMed:8276875 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-109 Sentence denotes Repair of O6-methylguanine and O4-methylthymine by the human and rat O6-methylguanine-DNA methyltransferases.
T2 110-418 Sentence denotes In order to compare the ability of the human and rat O6-methylguanine-DNA methyltransferases (transferases) to repair in vitro O6-methylguanine (O6-MeGua) and O4-methylthymine (O4-MeThy) residues, which are two mutagenic DNA adducts formed by alkylating agents, we have purified both proteins to homogeneity.
T3 419-582 Sentence denotes Gel electrophoresis of the proteins shows that the O4-MeThy repair is due to the transfer of the methyl group from the alkylated base to the transferase molecules.
T4 583-788 Sentence denotes However, both proteins repair with different efficiencies the O6-MeGua and O4-MeThy residues present in alkylated DNA, poly[d(G.C)], poly(dG.dC), or in alkylated poly[d(A.T)] and poly(dA.dT), respectively.
T5 789-879 Sentence denotes Reaction of both proteins with either methylated residues follows a second-order kinetics.
T6 880-1063 Sentence denotes The rate constants are 1 x 10(9) M-1 min-1 for both proteins acting on O6-MeGua and 4.8 x 10(6) or 1.8 x 10(5) M-1 min-1 for the rat or human protein acting on O4-MeThy, respectively.
T7 1064-1190 Sentence denotes The activity of the mammalian transferases on O4-MeThy present in a poly(dA.dT) substrate is inhibited by double-stranded DNA.
T1 0-109 Sentence denotes Repair of O6-methylguanine and O4-methylthymine by the human and rat O6-methylguanine-DNA methyltransferases.
T2 110-418 Sentence denotes In order to compare the ability of the human and rat O6-methylguanine-DNA methyltransferases (transferases) to repair in vitro O6-methylguanine (O6-MeGua) and O4-methylthymine (O4-MeThy) residues, which are two mutagenic DNA adducts formed by alkylating agents, we have purified both proteins to homogeneity.
T3 419-582 Sentence denotes Gel electrophoresis of the proteins shows that the O4-MeThy repair is due to the transfer of the methyl group from the alkylated base to the transferase molecules.
T4 583-788 Sentence denotes However, both proteins repair with different efficiencies the O6-MeGua and O4-MeThy residues present in alkylated DNA, poly[d(G.C)], poly(dG.dC), or in alkylated poly[d(A.T)] and poly(dA.dT), respectively.
T5 789-879 Sentence denotes Reaction of both proteins with either methylated residues follows a second-order kinetics.
T6 880-1063 Sentence denotes The rate constants are 1 x 10(9) M-1 min-1 for both proteins acting on O6-MeGua and 4.8 x 10(6) or 1.8 x 10(5) M-1 min-1 for the rat or human protein acting on O4-MeThy, respectively.
T7 1064-1190 Sentence denotes The activity of the mammalian transferases on O4-MeThy present in a poly(dA.dT) substrate is inhibited by double-stranded DNA.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 55-60 OrganismTaxon denotes human 9606
T2 65-68 OrganismTaxon denotes rat 10114|10116
T4 149-154 OrganismTaxon denotes human 9606
T5 159-162 OrganismTaxon denotes rat 10114|10116
T7 1009-1012 OrganismTaxon denotes rat 10114|10116
T9 1016-1021 OrganismTaxon denotes human 9606

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 702-706 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T3 716-720 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T5 745-749 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T7 762-766 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T9 1132-1136 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775