Using cloned sialyltransferases, Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3Gal I) and Gal beta 1,4GlcNAc alpha 2,6-sialyltransferase (ST6Gal I) from both chicken and mouse, CMP-NeuAc and CMP-NeuGc were compared as donor substrates with pyridylamino-oligo-saccharides as acceptors.