> top > docs > PubMed:29674318 > annotations

PubMed:29674318 JSONTXT

Annnotations TAB JSON ListView MergeView

PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 143-275 DRI_Background denotes ctosidases (EC 3.2.1.22) are retaining glycosidases that cleave terminal α-linked galactose residues from glycoconjugate substrates.
T2 276-326 DRI_Background denotes α-Galactosidases take part in the turnover of cell
T3 358-434 DRI_Background denotes in plants and in the lysosomal degradation of glycosphingolipids in animals.
T4 435-661 DRI_Background denotes Deficiency of human α-galactosidase A (α-Gal A) causes Fabry disease (FD), a heritable, X-linked lysosomal storage disorder, characterized by accumulation of globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3).
T5 730-894 DRI_Background denotes An activity-based probe (ABP) covalently labeling the catalytic nucleophile of α-Gal A has been previously designed to study α-galactosidases for use in FD therapy.
T6 895-1050 DRI_Approach denotes Here, we report that this ABP labels proteins in Nicotiana benthamiana leaf extracts, enabling the identification and biochemical characterization of an N.
T7 1079-1127 DRI_Approach denotes we name here A1.1 (gene accession ID GJZM-1660).
T8 1128-1322 DRI_Background denotes The transiently overexpressed and purified enzyme was a monomer lacking N-glycans and was active toward 4-methylumbelliferyl-α-d-galactopyranoside substrate (Km = 0.17 mm) over a broad pH range.
T9 1323-1518 DRI_Background denotes A1.1 structural analysis by X-ray crystallography revealed marked similarities with human α-Gal A, even including A1.1's ability to hydrolyze Gb3 and lyso-Gb3, which are not endogenous in plants.
T10 1519-1691 DRI_Approach denotes Of note, A1.1 uptake into FD fibroblasts reduced the elevated lyso-Gb3 levels in these cells, consistent with A1.1 delivery to lysosomes as revealed by confocal microscopy.
T11 1692-1952 DRI_Outcome denotes The ease of production and the features of A1.1, such as stability over a broad pH range, combined with its capacity to degrade glycosphingolipid substrates, warrant further examination of its value as a potential therapeutic agent for ERT-based FD management.

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 225-234 Glycan_Motif denotes galactose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE
T3 343-357 Glycan_Motif denotes galactomannans https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G93424OB
T4 593-614 Glycan_Motif denotes globotriaosylceramide https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T5 616-619 Glycan_Motif denotes Gb3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T6 656-659 Glycan_Motif denotes Gb3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T7 1465-1468 Glycan_Motif denotes Gb3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T8 1478-1481 Glycan_Motif denotes Gb3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T9 1586-1589 Glycan_Motif denotes Gb3 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 225-234 https://glytoucan.org/Structures/Glycans/G65889KE denotes galactose
T2 225-234 https://glytoucan.org/Structures/Glycans/G68158BT denotes galactose
T3 343-357 https://glytoucan.org/Structures/Glycans/G93424OB denotes galactomannans
T4 593-614 https://glytoucan.org/Structures/Glycans/G00059MO denotes globotriaosylceramide
T5 616-619 https://glytoucan.org/Structures/Glycans/G00059MO denotes Gb3
T6 656-659 https://glytoucan.org/Structures/Glycans/G00059MO denotes Gb3
T7 1465-1468 https://glytoucan.org/Structures/Glycans/G00059MO denotes Gb3
T8 1478-1481 https://glytoucan.org/Structures/Glycans/G00059MO denotes Gb3
T9 1586-1589 https://glytoucan.org/Structures/Glycans/G00059MO denotes Gb3

sentences

Id Subject Object Predicate Lexical cue
T1 0-136 Sentence denotes Nicotiana benthamiana α-galactosidase A1.1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease.
T2 137-434 Sentence denotes α-Galactosidases (EC 3.2.1.22) are retaining glycosidases that cleave terminal α-linked galactose residues from glycoconjugate substrates. α-Galactosidases take part in the turnover of cell wall-associated galactomannans in plants and in the lysosomal degradation of glycosphingolipids in animals.
T3 435-661 Sentence denotes Deficiency of human α-galactosidase A (α-Gal A) causes Fabry disease (FD), a heritable, X-linked lysosomal storage disorder, characterized by accumulation of globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3).
T4 662-729 Sentence denotes Current management of FD involves enzyme-replacement therapy (ERT).
T5 730-894 Sentence denotes An activity-based probe (ABP) covalently labeling the catalytic nucleophile of α-Gal A has been previously designed to study α-galactosidases for use in FD therapy.
T6 895-1127 Sentence denotes Here, we report that this ABP labels proteins in Nicotiana benthamiana leaf extracts, enabling the identification and biochemical characterization of an N. benthamiana α-galactosidase we name here A1.1 (gene accession ID GJZM-1660).
T7 1128-1322 Sentence denotes The transiently overexpressed and purified enzyme was a monomer lacking N-glycans and was active toward 4-methylumbelliferyl-α-d-galactopyranoside substrate (Km = 0.17 mm) over a broad pH range.
T8 1323-1518 Sentence denotes A1.1 structural analysis by X-ray crystallography revealed marked similarities with human α-Gal A, even including A1.1's ability to hydrolyze Gb3 and lyso-Gb3, which are not endogenous in plants.
T9 1519-1691 Sentence denotes Of note, A1.1 uptake into FD fibroblasts reduced the elevated lyso-Gb3 levels in these cells, consistent with A1.1 delivery to lysosomes as revealed by confocal microscopy.
T10 1692-1952 Sentence denotes The ease of production and the features of A1.1, such as stability over a broad pH range, combined with its capacity to degrade glycosphingolipid substrates, warrant further examination of its value as a potential therapeutic agent for ERT-based FD management.

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue glyco_epitope_db_id
T1 616-619 GlycoEpitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T2 656-659 GlycoEpitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T3 1465-1468 GlycoEpitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T4 1478-1481 GlycoEpitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T5 1586-1589 GlycoEpitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 343-357 Glycan denotes galactomannans https://api.glycosmos.org/wurcs2image/latest/png/binary/G93424OB
T2 616-619 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO
T3 656-659 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO
T4 1465-1468 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO
T5 1478-1481 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO
T6 1586-1589 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 122-135 Disease denotes Fabry disease http://purl.obolibrary.org/obo/MONDO_0010526
T2 490-503 Disease denotes Fabry disease http://purl.obolibrary.org/obo/MONDO_0010526
T3 505-507 Disease denotes FD http://purl.obolibrary.org/obo/MONDO_0010526
T4 532-558 Disease denotes lysosomal storage disorder http://purl.obolibrary.org/obo/MONDO_0002561
T5 684-686 Disease denotes FD http://purl.obolibrary.org/obo/MONDO_0010526
T6 883-885 Disease denotes FD http://purl.obolibrary.org/obo/MONDO_0010526
T7 1545-1547 Disease denotes FD http://purl.obolibrary.org/obo/MONDO_0010526
T8 1938-1940 Disease denotes FD http://purl.obolibrary.org/obo/MONDO_0010526

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 616-619 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO
T2 656-659 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO
T3 1465-1468 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO
T4 1478-1481 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO
T5 1586-1589 Glycan denotes Gb3 https://api.glycosmos.org/wurcs2image/latest/png/binary/G00059MO

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 616-619 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T2 656-659 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T3 1465-1468 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T4 1478-1481 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T5 1586-1589 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071

GlyCosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 1548-1559 Cell denotes fibroblasts http://purl.obolibrary.org/obo/CL:0000057

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 327-331 Body_part denotes wall http://purl.obolibrary.org/obo/UBERON_0000060
T2 1548-1559 Body_part denotes fibroblasts http://purl.obolibrary.org/obo/CL_0000057
T3 1646-1655 Body_part denotes lysosomes http://purl.obolibrary.org/obo/GO_0005764

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 122-135 Disease denotes Fabry disease MONDO:0010526
T2 490-503 Disease denotes Fabry disease MONDO:0010526
T3 505-507 Disease denotes FD MONDO:0010526
T4 532-558 Disease denotes lysosomal storage disorder MONDO:0002561
T5 684-686 Disease denotes FD MONDO:0010526
T6 883-885 Disease denotes FD MONDO:0010526
T7 1545-1547 Disease denotes FD MONDO:0010526
T8 1938-1940 Disease denotes FD MONDO:0010526

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 0-21 Organism denotes Nicotiana benthamiana 4100
T2 71-76 Organism denotes human 9606
T3 449-454 Organism denotes human 9606
T4 944-965 Organism denotes Nicotiana benthamiana 4100
T5 1407-1412 Organism denotes human 9606

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 616-619 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T2 656-659 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T3 1465-1468 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T4 1478-1481 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071
T5 1586-1589 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Gb3 http://www.glycoepitope.jp/epitopes/EP0071

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-136 Sentence denotes Nicotiana benthamiana α-galactosidase A1.1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease.
T2 137-434 Sentence denotes α-Galactosidases (EC 3.2.1.22) are retaining glycosidases that cleave terminal α-linked galactose residues from glycoconjugate substrates. α-Galactosidases take part in the turnover of cell wall-associated galactomannans in plants and in the lysosomal degradation of glycosphingolipids in animals.
T3 435-661 Sentence denotes Deficiency of human α-galactosidase A (α-Gal A) causes Fabry disease (FD), a heritable, X-linked lysosomal storage disorder, characterized by accumulation of globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3).
T4 662-729 Sentence denotes Current management of FD involves enzyme-replacement therapy (ERT).
T5 730-894 Sentence denotes An activity-based probe (ABP) covalently labeling the catalytic nucleophile of α-Gal A has been previously designed to study α-galactosidases for use in FD therapy.
T6 895-1127 Sentence denotes Here, we report that this ABP labels proteins in Nicotiana benthamiana leaf extracts, enabling the identification and biochemical characterization of an N. benthamiana α-galactosidase we name here A1.1 (gene accession ID GJZM-1660).
T7 1128-1322 Sentence denotes The transiently overexpressed and purified enzyme was a monomer lacking N-glycans and was active toward 4-methylumbelliferyl-α-d-galactopyranoside substrate (Km = 0.17 mm) over a broad pH range.
T8 1323-1518 Sentence denotes A1.1 structural analysis by X-ray crystallography revealed marked similarities with human α-Gal A, even including A1.1's ability to hydrolyze Gb3 and lyso-Gb3, which are not endogenous in plants.
T9 1519-1691 Sentence denotes Of note, A1.1 uptake into FD fibroblasts reduced the elevated lyso-Gb3 levels in these cells, consistent with A1.1 delivery to lysosomes as revealed by confocal microscopy.
T10 1692-1952 Sentence denotes The ease of production and the features of A1.1, such as stability over a broad pH range, combined with its capacity to degrade glycosphingolipid substrates, warrant further examination of its value as a potential therapeutic agent for ERT-based FD management.

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 379-388 Body_part denotes lysosomal FMA:63836
T2 532-541 Body_part denotes lysosomal FMA:63836
T3 1548-1559 Body_part denotes fibroblasts FMA:63877
T4 1646-1655 Body_part denotes lysosomes FMA:63836

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 0-21 OrganismTaxon denotes Nicotiana benthamiana 4100
T2 71-76 OrganismTaxon denotes human 9606
T3 449-454 OrganismTaxon denotes human 9606
T4 944-965 OrganismTaxon denotes Nicotiana benthamiana 4100
T5 1407-1412 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 327-331 Body_part denotes wall http://purl.obolibrary.org/obo/UBERON_0000060
T2 1548-1559 Body_part denotes fibroblasts http://purl.obolibrary.org/obo/CL_0000057
T3 1646-1655 Body_part denotes lysosomes http://purl.obolibrary.org/obo/GO_0005764

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 1548-1559 Cell denotes fibroblasts http://purl.obolibrary.org/obo/CL:0000057