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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 133-384 BACKGROUND denotes We aimed to determine and compare the in vitro effects of autologous bone marrow-derived mesenchymal stem cells (BM-MSCs) and mesenchymal stem cell supernatant (MSC-Sp) on the wound healing capacity of equine corneal fibroblasts using a scratch assay.
T2 394-1296 METHODS denotes Bone marrow aspirates and eyes were collected from normal, euthanized horses with subsequent isolation and culture of BM-MSCs and corneal stromal cells. Corneal stromal cells were culture-expanded in the culture well of transwell plates and then treated with an autologous BM-MSC suspension (dose: 2.5 × 10(5)/100 μL media with the BM-MSCs contained within the insert well), MSC-Sp solution, or naive culture media (control) for 72 h. A linear defect in confluent cell cultures was created (i.e., corneal scratch assay) to assess the cellular closure ("healing") over time. Three representative areas of the scratch in each culture were photographed at each time point and the scratch area was quantitated using image analysis software (ImageJ). Media from the scratches were analyzed for various growth factors using human enzyme-linked immunosorbent assay (ELISA) kits that crossreact with the horse.
T3 1306-1769 RESULTS denotes There was a significant percentage decrease in the scratch area remaining in the BM-MSC and MSC-Sp groups compared to the control group. There was also a significant percentage decrease in the scratch area remaining in the BM-MSC group compared to the MSC-Sp group at 36 h post-scratch and all time points thereafter. The concentration of transforming growth factor (TGF)-β1 in the media was significantly higher in the BM-MSC group compared to the control group.
T4 1783-2215 CONCLUSIONS denotes The significant decrease in scratch area in equine corneal fibroblast cultures treated with autologous BM-MSCs compared to MSC-Sp or control treatments suggests that BM-MSCs may substantially improve corneal wound healing in horses. MSC-Sp may also improve corneal wound healing given the significant decrease in scratch area compared to control treatments, and would be an immediately available and cost-effective treatment option.

Goldhamster2_Cellosaurus

Id Subject Object Predicate Lexical cue
T1 10-14 CVCL_D953|Induced_pluripotent_stem_cell|Homo sapiens denotes bone
T2 133-135 CVCL_5M23|Cancer_cell_line|Mesocricetus auratus denotes We
T3 202-206 CVCL_D953|Induced_pluripotent_stem_cell|Homo sapiens denotes bone
T4 246-248 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T5 246-248 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T6 298-300 CVCL_1Y11|Transformed_cell_line|Homo sapiens denotes Sp
T7 298-300 CVCL_L986|Undefined_cell_line_type|Aotus trivirgatus denotes Sp
T8 368-369 CVCL_6479|Finite_cell_line|Mus musculus denotes a
T9 394-398 CVCL_D953|Induced_pluripotent_stem_cell|Homo sapiens denotes Bone
T10 464-470 CVCL_9124|Undefined_cell_line_type|Cricetulus griseus denotes horses
T11 512-514 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T12 512-514 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T13 624-630 CVCL_B488|Transformed_cell_line|Homo sapiens denotes plates
T14 653-655 CVCL_8754|Cancer_cell_line|Homo sapiens denotes an
T15 653-655 CVCL_H241|Cancer_cell_line|Homo sapiens denotes an
T16 667-669 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T17 667-669 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T18 726-728 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T19 726-728 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T20 773-775 CVCL_1Y11|Transformed_cell_line|Homo sapiens denotes Sp
T21 773-775 CVCL_L986|Undefined_cell_line_type|Aotus trivirgatus denotes Sp
T22 829-830 CVCL_6479|Finite_cell_line|Mus musculus denotes A
T23 962-966 CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens denotes time
T24 1052-1056 CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens denotes time
T25 1290-1295 CVCL_9124|Undefined_cell_line_type|Cricetulus griseus denotes horse
T26 1316-1317 CVCL_6479|Finite_cell_line|Mus musculus denotes a
T27 1387-1389 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T28 1387-1389 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T29 1402-1404 CVCL_1Y11|Transformed_cell_line|Homo sapiens denotes Sp
T30 1402-1404 CVCL_L986|Undefined_cell_line_type|Aotus trivirgatus denotes Sp
T31 1458-1459 CVCL_6479|Finite_cell_line|Mus musculus denotes a
T32 1529-1531 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T33 1529-1531 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T34 1562-1564 CVCL_1Y11|Transformed_cell_line|Homo sapiens denotes Sp
T35 1562-1564 CVCL_L986|Undefined_cell_line_type|Aotus trivirgatus denotes Sp
T36 1574-1576 CVCL_9087|Hybrid_cell_line|Homo sapiens,Mus musculus denotes 36
T37 1600-1604 CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens denotes time
T38 1726-1728 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T39 1726-1728 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T40 1886-1888 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T41 1886-1888 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T42 1910-1912 CVCL_1Y11|Transformed_cell_line|Homo sapiens denotes Sp
T43 1910-1912 CVCL_L986|Undefined_cell_line_type|Aotus trivirgatus denotes Sp
T44 1949-1951 CVCL_E481|Transformed_cell_line|Homo sapiens denotes BM
T45 1949-1951 CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer denotes BM
T46 1957-1960 CVCL_E773|Transformed_cell_line|Homo sapiens denotes may
T47 2008-2014 CVCL_9124|Undefined_cell_line_type|Cricetulus griseus denotes horses
T48 2020-2022 CVCL_1Y11|Transformed_cell_line|Homo sapiens denotes Sp
T49 2020-2022 CVCL_L986|Undefined_cell_line_type|Aotus trivirgatus denotes Sp
T50 2023-2026 CVCL_E773|Transformed_cell_line|Homo sapiens denotes may
T51 2154-2156 CVCL_8754|Cancer_cell_line|Homo sapiens denotes an
T52 2154-2156 CVCL_H241|Cancer_cell_line|Homo sapiens denotes an
T53 2183-2187 CVCL_9491|Cancer_cell_line|Homo sapiens denotes cost

GoldHamster

Id Subject Object Predicate Lexical cue
T4 10-21 UBERON:0002371 denotes bone marrow
T3 10-21 D001855 denotes bone marrow
T5 10-21 D001855 denotes bone marrow
T6 57-66 CL:0000034 denotes stem cell
T7 82-88 9796 denotes equine
T8 97-110 GO:0042060 denotes wound healing
T12 202-213 UBERON:0002371 denotes bone marrow
T11 202-213 D001855 denotes bone marrow
T13 202-213 D001855 denotes bone marrow
T15 249-253 PR:000023297 denotes MSCs
T16 249-253 PR:P0C0S1 denotes MSCs
T14 249-253 GO:0043854 denotes MSCs
T17 259-280 CL:0000134 denotes mesenchymal stem cell
T22 294-297 CL:0000134 denotes MSC
T19 294-297 PR:000010664 denotes MSC
T20 294-297 PR:O60682 denotes MSC
T21 294-297 PR:O88940 denotes MSC
T23 309-322 GO:0042060 denotes wound healing
T24 335-341 9796 denotes equine
T26 394-405 UBERON:0002371 denotes Bone marrow
T25 394-405 D001855 denotes Bone marrow
T27 394-405 D001855 denotes Bone marrow
T29 515-519 PR:000023297 denotes MSCs
T30 515-519 PR:P0C0S1 denotes MSCs
T28 515-519 GO:0043854 denotes MSCs
T31 670-673 PR:000010664 denotes MSC
T32 670-673 PR:O60682 denotes MSC
T33 670-673 PR:O88940 denotes MSC
T34 670-673 CL:0000134 denotes MSC
T36 729-733 PR:000023297 denotes MSCs
T37 729-733 PR:P0C0S1 denotes MSCs
T35 729-733 GO:0043854 denotes MSCs
T38 755-761 SO:0000046 denotes insert
T39 769-772 PR:000010664 denotes MSC
T40 769-772 PR:O60682 denotes MSC
T41 769-772 PR:O88940 denotes MSC
T42 769-772 CL:0000134 denotes MSC
T43 776-784 CHEBI:75958 denotes solution
T44 795-808 D003470 denotes culture media
T45 831-837 SO:0000987 denotes linear
T46 1191-1205 D036341 denotes growth factors
T47 1191-1205 D036341 denotes growth factors
T48 1212-1217 D006801 denotes human
T49 1290-1295 D006736 denotes horse
T50 1290-1295 9796 denotes horse
T51 1290-1295 CVCL_9124 denotes horse
T52 1390-1393 PR:000010664 denotes MSC
T53 1390-1393 PR:O60682 denotes MSC
T54 1390-1393 PR:O88940 denotes MSC
T55 1390-1393 CL:0000134 denotes MSC
T56 1398-1401 PR:000010664 denotes MSC
T57 1398-1401 PR:O60682 denotes MSC
T58 1398-1401 PR:O88940 denotes MSC
T59 1398-1401 CL:0000134 denotes MSC
T60 1532-1535 PR:000010664 denotes MSC
T61 1532-1535 PR:O60682 denotes MSC
T62 1532-1535 PR:O88940 denotes MSC
T63 1532-1535 CL:0000134 denotes MSC
T64 1558-1561 PR:000010664 denotes MSC
T65 1558-1561 PR:O60682 denotes MSC
T66 1558-1561 PR:O88940 denotes MSC
T67 1558-1561 CL:0000134 denotes MSC
T68 1645-1671 D015291 denotes transforming growth factor
T69 1645-1671 D015291 denotes transforming growth factor
T70 1658-1671 P08441 denotes growth factor
T71 1658-1671 P08072 denotes growth factor
T72 1658-1671 Q776B5 denotes growth factor
T73 1658-1671 Q6RZT5 denotes growth factor
T74 1658-1671 Q8V307 denotes growth factor
T78 1729-1732 CL:0000134 denotes MSC
T75 1729-1732 PR:000010664 denotes MSC
T76 1729-1732 PR:O60682 denotes MSC
T77 1729-1732 PR:O88940 denotes MSC
T79 1827-1833 9796 denotes equine
T80 1834-1852 CL:0002363 denotes corneal fibroblast
T83 1889-1893 PR:000023297 denotes MSCs
T84 1889-1893 PR:P0C0S1 denotes MSCs
T82 1889-1893 GO:0043854 denotes MSCs
T88 1906-1909 CL:0000134 denotes MSC
T85 1906-1909 PR:000010664 denotes MSC
T86 1906-1909 PR:O60682 denotes MSC
T87 1906-1909 PR:O88940 denotes MSC
T90 1952-1956 PR:000023297 denotes MSCs
T91 1952-1956 PR:P0C0S1 denotes MSCs
T89 1952-1956 GO:0043854 denotes MSCs
T92 1991-2004 GO:0042060 denotes wound healing
T93 2016-2019 PR:000010664 denotes MSC
T94 2016-2019 PR:O60682 denotes MSC
T95 2016-2019 PR:O88940 denotes MSC
T96 2016-2019 CL:0000134 denotes MSC
T97 2048-2061 GO:0042060 denotes wound healing