PubMed:27843266 / 0-693 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":108,"end":293},"obj":"PURPOSE"}],"text":"Heat shock protein concentration and clarity of porcine lenses incubated at elevated temperatures.\nPURPOSE: To quantify the concentration of heat shock proteins in lenses in lens organ culture at elevated temperatures, and to examine the relation between elevated temperature and lens clarity.\nMETHODS: Pig lenses obtained from a local abattoir were dissected aseptically and incubated in medium M199 without serum for 4 days to stabilize, and lenses with protein leakage of less than 10 mg/l were obtained for heat shock exposure. Heat shock was performed by incubation for 1 h in M199 without serum at various temperatures ranging from 37 °C to 55 °C. After incubation for 24 h, cataract blu"}

{"project":"Goldhamster2_Cellosaurus","denotations":[{"id":"T1","span":{"begin":328,"end":329},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T2","span":{"begin":575,"end":578},"obj":"CVCL_J416|Transformed_cell_line|Mus musculus"},{"id":"T3","span":{"begin":638,"end":646},"obj":"CVCL_R881|Spontaneously_immortalized_cell_line|Oreochromis mossambicus x Oreochromis niloticus"},{"id":"T4","span":{"begin":642,"end":643},"obj":"CVCL_S361|Finite_cell_line|Mus musculus"},{"id":"T5","span":{"begin":651,"end":652},"obj":"CVCL_S361|Finite_cell_line|Mus musculus"},{"id":"T6","span":{"begin":675,"end":679},"obj":"CVCL_8262|Cancer_cell_line|Homo sapiens"}],"text":"Heat shock protein concentration and clarity of porcine lenses incubated at elevated temperatures.\nPURPOSE: To quantify the concentration of heat shock proteins in lenses in lens organ culture at elevated temperatures, and to examine the relation between elevated temperature and lens clarity.\nMETHODS: Pig lenses obtained from a local abattoir were dissected aseptically and incubated in medium M199 without serum for 4 days to stabilize, and lenses with protein leakage of less than 10 mg/l were obtained for heat shock exposure. Heat shock was performed by incubation for 1 h in M199 without serum at various temperatures ranging from 37 °C to 55 °C. After incubation for 24 h, cataract blu"}

{"project":"GoldHamster","denotations":[{"id":"T1","span":{"begin":0,"end":18},"obj":"D006360"},{"id":"T6","span":{"begin":11,"end":18},"obj":"SO:0000104"},{"id":"T5","span":{"begin":11,"end":18},"obj":"PR:000000001"},{"id":"T2","span":{"begin":11,"end":18},"obj":"GO:0003675"},{"id":"T7","span":{"begin":56,"end":62},"obj":"UBERON:0000965"},{"id":"T8","span":{"begin":141,"end":160},"obj":"D006360"},{"id":"T10","span":{"begin":164,"end":170},"obj":"UBERON:0000965"},{"id":"T11","span":{"begin":174,"end":178},"obj":"UBERON:0000965"},{"id":"T12","span":{"begin":174,"end":178},"obj":"UBERON:0005389"},{"id":"T13","span":{"begin":179,"end":184},"obj":"UBERON:0000062"},{"id":"T14","span":{"begin":179,"end":184},"obj":"UBERON:0003103"},{"id":"T15","span":{"begin":280,"end":284},"obj":"UBERON:0000965"},{"id":"T16","span":{"begin":280,"end":284},"obj":"UBERON:0005389"},{"id":"T17","span":{"begin":303,"end":306},"obj":"9823"},{"id":"T18","span":{"begin":303,"end":306},"obj":"P30941"},{"id":"T19","span":{"begin":307,"end":313},"obj":"UBERON:0000965"},{"id":"T20","span":{"begin":409,"end":414},"obj":"UBERON:0001977"},{"id":"T21","span":{"begin":444,"end":450},"obj":"UBERON:0000965"},{"id":"T26","span":{"begin":456,"end":463},"obj":"SO:0000104"},{"id":"T25","span":{"begin":456,"end":463},"obj":"PR:000000001"},{"id":"T23","span":{"begin":456,"end":463},"obj":"CHEBI:36080"},{"id":"T24","span":{"begin":456,"end":463},"obj":"CHEBI:11122"},{"id":"T22","span":{"begin":456,"end":463},"obj":"GO:0003675"},{"id":"T28","span":{"begin":577,"end":581},"obj":"CHEBI:13359"},{"id":"T29","span":{"begin":595,"end":600},"obj":"UBERON:0001977"},{"id":"T30","span":{"begin":675,"end":679},"obj":"CVCL_8262"},{"id":"T31","span":{"begin":681,"end":689},"obj":"D002386"},{"id":"T32","span":{"begin":681,"end":689},"obj":"D002386"}],"text":"Heat shock protein concentration and clarity of porcine lenses incubated at elevated temperatures.\nPURPOSE: To quantify the concentration of heat shock proteins in lenses in lens organ culture at elevated temperatures, and to examine the relation between elevated temperature and lens clarity.\nMETHODS: Pig lenses obtained from a local abattoir were dissected aseptically and incubated in medium M199 without serum for 4 days to stabilize, and lenses with protein leakage of less than 10 mg/l were obtained for heat shock exposure. Heat shock was performed by incubation for 1 h in M199 without serum at various temperatures ranging from 37 °C to 55 °C. After incubation for 24 h, cataract blu"}