Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-122 |
Sentence |
denotes |
Metabolomics analysis identifies d-Alanine-d-Alanine ligase as the primary lethal target of d-Cycloserine in mycobacteria. |
T2 |
123-460 |
Sentence |
denotes |
d-Cycloserine is an effective second line antibiotic used as a last resort to treat multi (MDR)- and extensively (XDR) drug resistant strains of Mycobacterium tuberculosis . d-Cycloserine interferes with the formation of peptidoglycan biosynthesis by competitive inhibition of alanine racemase (Alr) and d-alanine-d-alanine ligase (Ddl). |
T3 |
461-556 |
Sentence |
denotes |
Although the two enzymes are known to be inhibited, the in vivo lethal target is still unknown. |
T4 |
557-715 |
Sentence |
denotes |
Our NMR metabolomics work has revealed that Ddl is the primary target of DCS, as cell growth is inhibited when the production of d-alanyl-d-alanine is halted. |
T5 |
716-872 |
Sentence |
denotes |
It is shown that inhibition of Alr may contribute indirectly by lowering the levels of d-alanine, thus allowing DCS to outcompete d-alanine for Ddl binding. |
T6 |
873-1028 |
Sentence |
denotes |
The NMR data also supports the possibility of a transamination reaction to produce d-alanine from pyruvate and glutamate, thereby bypassing Alr inhibition. |
T7 |
1029-1244 |
Sentence |
denotes |
Furthermore, the inhibition of peptidoglycan synthesis results in a cascading effect on cellular metabolism as there is a shift toward the catabolic routes to compensate for accumulation of peptidoglycan precursors. |
T1 |
0-122 |
Sentence |
denotes |
Metabolomics analysis identifies d-Alanine-d-Alanine ligase as the primary lethal target of d-Cycloserine in mycobacteria. |
T2 |
123-460 |
Sentence |
denotes |
d-Cycloserine is an effective second line antibiotic used as a last resort to treat multi (MDR)- and extensively (XDR) drug resistant strains of Mycobacterium tuberculosis . d-Cycloserine interferes with the formation of peptidoglycan biosynthesis by competitive inhibition of alanine racemase (Alr) and d-alanine-d-alanine ligase (Ddl). |
T3 |
461-556 |
Sentence |
denotes |
Although the two enzymes are known to be inhibited, the in vivo lethal target is still unknown. |
T4 |
557-715 |
Sentence |
denotes |
Our NMR metabolomics work has revealed that Ddl is the primary target of DCS, as cell growth is inhibited when the production of d-alanyl-d-alanine is halted. |
T5 |
716-872 |
Sentence |
denotes |
It is shown that inhibition of Alr may contribute indirectly by lowering the levels of d-alanine, thus allowing DCS to outcompete d-alanine for Ddl binding. |
T6 |
873-1028 |
Sentence |
denotes |
The NMR data also supports the possibility of a transamination reaction to produce d-alanine from pyruvate and glutamate, thereby bypassing Alr inhibition. |
T7 |
1029-1244 |
Sentence |
denotes |
Furthermore, the inhibition of peptidoglycan synthesis results in a cascading effect on cellular metabolism as there is a shift toward the catabolic routes to compensate for accumulation of peptidoglycan precursors. |