PubMed:22768869 JSONTXT

{"project":"Allie","denotations":[{"id":"SS1_22768869_2_0","span":{"begin":137,"end":168},"obj":"expanded"},{"id":"SS2_22768869_2_0","span":{"begin":170,"end":174},"obj":"abbr"},{"id":"SS1_22768869_6_0","span":{"begin":719,"end":738},"obj":"expanded"},{"id":"SS2_22768869_6_0","span":{"begin":739,"end":744},"obj":"abbr"}],"relations":[{"id":"AE1_22768869_2_0","pred":"abbreviatedTo","subj":"SS1_22768869_2_0","obj":"SS2_22768869_2_0"},{"id":"AE1_22768869_6_0","pred":"abbreviatedTo","subj":"SS1_22768869_6_0","obj":"SS2_22768869_6_0"}],"text":"[Optimization of magnetic activated cell sorting and the biological characteristics of isolated cells].\nAIM: To optimize the traditional magnetic activated cell sorting (MACS) so as to enhance the purification and keep the viability of cells after separation.\nMETHODS: Take stem cell antigen-1 labeled hemopoietic stem/progenitor cells (Sca-1(+);HSC/HPC) for example. The traditional MACS and the optimized MACS were applied to obtain the Sca-1(+); HSC/HPC from mouse bone marrow respectively. The purifications of Sca-1(+); cells from two groups were tested by flow cytometry; The survival rates of Sca-1(+); cells from two groups were detected by trypan blue dye; The proliferation of Sca-1(+); cells was detected by cell counting kit-8(CCK-8); the differentiation capacity of Sca-1(+); cells was measured by CFU-Mix.\nRESULTS: The purification of Sca-1(+); cells was up to 93% by the optimized MACS compared to 87% by the traditional one; the recovery rate of positive cells was 73% by the optimized MACS compared to 62.3% by the traditional one; there was no statistical difference in the viability and proliferation of Sca-1(+); cells and the capacity of producing CFU-Mix between two groups.\nCONCLUSION: The optimized MACS can significantly enhance the purification and recovery rate of Sca-1(+); cells without influence of cell viability and biological features, which can be considered to apply in other MACS."}