PubMed:1797400 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1797400","sourcedb":"PubMed","sourceid":"1797400","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1797400","text":"Relationship between conformation and biological response for (1----3)-beta-D-glucans in the activation of coagulation factor G from limulus amebocyte lysate and host-mediated antitumor activity. Demonstration of single-helix conformation as a stimulant.\nThe relationship between the conformation of (1----3)-beta-D-glucans in gel or hydrated form and the stimulation of two types of biological responses, namely, activation of coagulation Factor G from limulus amebocyte lysate (LAL) and host-mediated antitumor activity was examined. Both types were activated by the single-helical conformation, as revealed by high-resolution, solid-state 13C-n.m.r. spectroscopy. The potency of activation of Factor G was increased over 100-fold by treatment with a NaOH solution which leads to a complete or partial conversion from the triple to the single helix. Such a single-helix specific response was also demonstrated for the antitumor activity of curdlan, although the distinction was less pronounced for branched (1----3)-beta-D-glucans. The presence of the single-helix conformation was observed in schizophyllan gel, even though the triple helix is the most stable form of branched glucans in aqueous media.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":195},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":196,"end":254},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":255,"end":535},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":536,"end":666},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":667,"end":851},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":852,"end":1033},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1034,"end":1205},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":195},"obj":"Sentence"},{"id":"T2","span":{"begin":196,"end":254},"obj":"Sentence"},{"id":"T3","span":{"begin":255,"end":535},"obj":"Sentence"},{"id":"T4","span":{"begin":536,"end":666},"obj":"Sentence"},{"id":"T5","span":{"begin":667,"end":851},"obj":"Sentence"},{"id":"T6","span":{"begin":852,"end":1033},"obj":"Sentence"},{"id":"T7","span":{"begin":1034,"end":1205},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":133,"end":140},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":454,"end":461},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"6849"},{"id":"A2","pred":"db_id","subj":"T2","obj":"6849"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":220,"end":225},"obj":"Body_part"},{"id":"T2","span":{"begin":845,"end":850},"obj":"Body_part"},{"id":"T3","span":{"begin":866,"end":871},"obj":"Body_part"},{"id":"T4","span":{"begin":1061,"end":1066},"obj":"Body_part"},{"id":"T5","span":{"begin":1138,"end":1143},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T4","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T5","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#e093ec","default":true},{"id":"NCBITAXON","color":"#93ecc6"},{"id":"Anatomy-UBERON","color":"#ecac93"}]}]}}