Analysis of restriction fingerprints derived from single clones – single clone analysis (SCA) A computer program (written with the Microsoft Visual Studio 6 software suite) was designed that extracted fingerprints from the raw data of single clone analysis. In one lane, the peak for each restriction enzyme was chosen, that had the maximum height (NewHeight) after applying the following empirically determined formula in order to account for the broadening in peak shape and the resulting lower peak height that goes along with increasing numbers of base pairs: NewHeight = MeasuredHeight / ((CalculatedBasePairs / - Denominator) + Addend), with Denominator = 3.0, Addend = 500. The corresponding calculated lengths of the fragments were stored as fingerprints in the database.