PMC:99051 / 23826-24484 JSONTXT

{"project":"2_test","denotations":[{"id":"11882253-1538749-10250608","span":{"begin":477,"end":479},"obj":"1538749"},{"id":"11882253-7570003-10250609","span":{"begin":565,"end":567},"obj":"7570003"}],"text":"In conclusion, DADA is a digital method for analyzing expression levels based on the counting of restriction fingerprints of cDNA clones. It is built around a specifically designed cloning vector that suppresses misleading fingerprints derived from partial cDNAs or empty vectors. The method yields quantitative data and absolute figures that do not depend on amplification by polymerase chain reaction. Compared to the sequencing of cDNA libraries (conventional EST approach [14]), it is substantially faster and more economical. In comparison to the SAGE method [20] it results in physical cDNA clones of substantial length which speed up further analysis."}