PMC:99051 / 14925-15533 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/99051","sourcedb":"PMC","sourceid":"99051","source_url":"https://www.ncbi.nlm.nih.gov/pmc/99051","text":"We randomly picked single clones of 96 colonies from a murine wounded skin library and compared the analyzed restriction fingerprints with the corresponding, experimentally determined cDNA sequence of the vectors. Vectors containing no cDNA inserts produced no signals in the detection window of 45 to 900 base pairs and incomplete or short cDNA inserts yielded only signals of fragments derived from restrictions within the cDNA sequence. This demonstrated that the restriction free region next to the cDNA cloning site fulfills its function in repressing misleading fragments and the corresponding signals.","tracks":[]}