Our studies investigated the viability and size distribution of particles created by a number of different nebuliser types; 3- and 6-jet Collison nebulisers; two medical nebulisers (normally used to deliver therapeutics) [23,24,25,26,27]; and two sparging liquid aerosol generators (1 inch and 90 mm SLAGs). In terms of nebuliser function, the “gold standard” Collison, created in 1973 [28], generates aerosols by applying an airflow (at around 26psi) to a liquid suspension of microorganisms; creating a vacuum which draws the suspension up a tube into which are engineered jets (between 1 and 24, depending on model type). The liquid suspension exits the jets at high force, creating particles which impact onto the surrounding glass jar and break up into smaller particles, for delivery into the test system. This method is designed to deliver near mono-disperse aerosols, in the size range of 1 to 3 µm [29]; but is considered “harsh” on microorganisms, applying both shearing and impaction forces which can render a proportion of the organisms non-viable. It also re-circulates the liquid suspension meaning that over longer spray times, the viability of the suspension can decrease. As SARS-CoV-2 is an enveloped virus, it is thought to be more prone to damage from the environment than unenveloped viruses. We therefore investigated nebulisers employing different aerosolization methods for comparison.