GCN/cm2 of BCoV at 25 °C and 60% and 80% relative humidity of the chamber swab, the wood, metal, and plastic surface swab samples (Table S2 in Supplementary Material). The results of BCoV association, Figure 9, and dissociation, Figure 10, to surfaces were obtained using the BLItz system. Figure 9 shows the association constant for wood, metal, and plastic surface swab samples at 60% and 80% relative humidity. The ka values of the wood, metal, and plastic swab samples are 8.733 × 106 Ms−1, 6.948 × 106 Ms−1, and 9.842 × 106 Ms−1 for 60% relative humidity and 7.749 × 106 Ms−1, 8.658 × 106 Ms−1, and 1.065 × 107 Ms−1 for 80% relative humidity, respectively. Figure 10 shows the dissociation constant for wood, metal, and plastic surface swab samples at 60% relative humidity and 80% relative humidity. The kd values of the wood, metal, and plastic swab samples are 1.302 × 10−4 s−1, 1.00 × 10−7 s−1, and 1.00 × 10−7 s−1 for 60% relative humidity and 3.071 × 10−6 s−1, 1.00 × 10−7 s−1, and 1.00 × 10−7 s−1 for 80% relative humidity, respectively. The ANOVA test was performed comparing the different surface swab samples ka values at 60% and 80% relative humidity returned p-values of 0.811 and 0.247. There was no significant difference between the ka values of the swab surface samples at 60% or 80% relative humidity. Based on the ANOVA tests performed for each swab sample comparing the 60% and 80% results, there was no significant difference between the ka at 60% and 80% relative humidity. The ANOVA test performed comparing the different surface swab samples at 60% and 80% relative humidity returned p-values of 5.25 × 10−6 and 0.422. There was significant difference between the kd values of the swab surface samples at 60% relative humidity but not at 80% relative humidity. The wood swab sample was the only surface sample with a significantly different kd value between the 60% and 80% relative humidity tests with a p-value of 2.59 × 10−4. The swab samples from metal and plastic exhibited stronger association at 80% relative humidity, though not with a significant difference. The swab sample from wood showed stronger dissociation at 80% relative humidity compared to 60%. The difference may be explained by the increased hydrophobicity of the PP (~18 MPa1/2) polymer compared to the lower value for PU (~24 MPa1/2) based on the Hansen model [36]. Polymers composed of exclusively hydrocarbon constituents (PP) are most hydrophobic, while PU may interact at higher humidity with the phosphate ions in the PBS buffer, resulting in the detachment of the virus particles from the surface. In a recent study with bovine coronavirus, the authors found increased attachment to hydrophobic surfaces [29].