Bio-layer interferometry (BLI) was performed on the surface swab samples to determine the kinetics of the samples collected for each surface under the different environmental conditions. BLI is a technique used to measure the micromolar interactions through white light interference caused by the sample attaching to the biosensor surface. The BLItz system (ForteBio, Fremont, CA, USA) was used for the testing with Aminopropylsilane (APS) biosensors (ForteBio, Fremont, CA, USA). It is important to understand the hydrophobic interaction between BCoV and the hydrophobic APS biosensor because the S protein of SARS-CoV-2 binds to the angiotensin-converting enzyme 2 (ACE2) receptors of its host cells. This binding interaction occurs in a hydrophobic region. The BLI results will show how the S protein would bind to human cell receptors after coming into contact with these 3 surfaces in a hospital [29,30]. Each biosensor was hydrated in Phosphate Buffer Saline (PBS) buffer (pH 7.4) for 10 min before use. The tests had 3 steps, baseline, association, and dissociation. The baseline step was performed with 4 μL of PBS buffer for 30 s. The association step used 4 μL of sample for 300 s to allow the sample to associate to the surface and measure the association of the sample to the biosensor surface. Dissociation took 300 s with 4 μL of PBS buffer to allow the sample to dissociate from the surface and measure the sample dissociation from the biosensor surface. The BLItz system was used to generate binding curves with local modeling using a designated reference. Based on the curve, the association and dissociation rates and kinetics constants were determined for each surface swab sample.