Traditionally, habitual dietary intake has been estimated using subjective recall data derived from food frequency questionnaires (FFQ). This approach, despite its direct translation for establishing dietary recommendations, does not account for recall bias, particularly among subjects with varied cognitive performance, and inter-individual variability in the nutrient absorption and metabolism (14). There has been an attempt to overcome these issues by constructing serum nutrient patterns (NP) as a marker of intake among subjects in the Oregon Brain Aging Study (15), and the Illinois Brain Aging Study (16–18). However, given that cognitive processes originate from the human brain, particularly in neocortices, and that nutrient uptake into the central nervous system (CNS) is strictly regulated at the blood-brain barrier (BBB) (19), we need to expand the scope of investigation further into the CNS for a greater apprehension of nutrition's roles in the aging brain.