The morphological state of loaded bacteria after disinfection treatments was analyzed by FE-SEM (Figure 6C). With microwaving, oven-dry, and UV irradiation, disruption of E. coli membrane integrity was observed; on the contrary, IPA immersion and water-laundering resulted in both cell deformation and cell detachment. Remaining bacteria on water-laundered media was observed in a tangled structure of cells with contaminants on the fiber surface. Generally, microwaving and oven-drying rely solely on the heating effect to inactivate microorganisms [52], as heating triggers the denaturation of cell proteins [53]. Additionally, the UV irradiation breaks the strong bond of cell membranes, such as O-H, P-O, and N-H bonds of E. coli, or induces oxidation, leading to cell death [54]. The solvents and detergent laundering treatments have compound effect of bactericidal and bacteria removal activity, by accumulating in the lipid of membrane and degrading the membrane integrity while inducing suspension of bacterial droplet into the liquid [55,56]. Meanwhile, laundering effectively removed bacteria by detaching them from fibers, rather than sterilizing the cells.