| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T89 |
0-200 |
Sentence |
denotes |
The present data demonstrate that the PASylation technology can solve two major problems of a peptide drug: (i) instability in the expression host and (ii) low bioactivity due to fast renal clearance. |
| T90 |
201-442 |
Sentence |
denotes |
Typically, small peptides, if expressed in a soluble state in E. coli, are quickly degraded by host cell proteases [42] and, thus, require production as larger fusion proteins as well as subsequent release by site-specific cleavage in vitro. |
| T91 |
443-685 |
Sentence |
denotes |
Here, fusion with a random-coil forming PAS sequence of ~600 amino acids prevented proteolytic degradation and allowed cost-efficient one-step production of the intact fusion protein in E. coli without the need of additional processing steps. |
