The 14 serological assays listed in Table 1 were performed on each of the 23 sera from PCR confirmed COVID-19 positive patients and the 19 pre-pandemic/cross-reactive sera, according to the manufacturer’s instructions. Sensitivity and specificity were calculated for each test. We calculated both the overall sensitivity (day 2–18 after symptom onset) and the sensitivity of the two subgroups (day 2–7 and day 8–18), each time for IgM/A and IgG separately and for combined IgM/A and IgG testing.
Table 1. Overview of the included assays
Test Assay Company Antibody detection Recombinant antigen
Point-of-care test
Corona Virus (COVID-19) Combined IgM/IgG Rapid Test Sol Scientifics IgM+IgG Not specified
COVID-19 IgG/igM Rapid Test Cassette Zhejiang ORIENT GENE Biotech IgM+IgG Spike + nucleocapsid protein
Wantai SARS-COV-2 Ab Rapid Test Beijing Wantai Biological Pharmacy Enterprise IgM+IgG (combined) Not specified
COVID-19 IgG/IgM RAPID TEST PRIMA PROFESSIONAL IgM+IgG Not specified
Diagnostic Kit for IgG/IgM Antibody to SARS-CoV-2 WIZ BIOTECH IgM+IgG Not specified
Enzyme-Linked Immunosorbent Assay (ELISA)
Anti-SARS-CoV-2 ELISA (IgA) EUROIMMUN S1 Spike glycoprotein (S1domain)
Anti-SARS-CoV-2 ELISA (IgG) EUROIMMUN IgG Spike glycoprotein (S1domain)
Novel Coronavirus COVID-19 IgM ELISA Kit Epitope Diagnostics (EDI) IgM Not specified
Novel Coronavirus COVID-19 IgG ELISA Kit Epitope Diagnostics (EDI) IgG Nucleocapsid protein
SARS-CoV-2 IgM ELISA Kit Creative Diagnostics IgM Not specified
SARS-CoV-2 IgG ELISA kit Creative Diagnostics IgG Not specified
Human Anti-2019 nCoV(N) IgG ELISA kit Finetest, Wuhan Fine Biotech Co., Ltd. IgG Spike glycoprotein (S1domain)
Chemiluminescent microparticle immunoassay (CMIA)
SARS-CoV-2 IgG Abbott IgG Nucleocapsid protein
Chemiluminescence immunoassay (CLIA)
Liaison SARS-CoV-2 S1/S2 IgG DiaSorin IgG S1 and S2 protein