Table 4  Comparison of sensitivity between RT-qPCR and RT-LAMP assays.
Dilution rate of viral RNA
10−2  10−3  10−4  10−5  Water
RT-LAMP  ORF1b_1  Time (min)  6.5  7.8  9.0  -  -
6.3  7.3  9.0  9.5  -
6.5  7.8  12.8  -  -
Average  6.4  7.6  10.3  -  -
SD  0.1  0.2  1.8  -  -
Positive  3/3  3/3  3/3  1/3  0/3
ORF1b_2  Time (min)  6.8  7.5  9.5  -  -
6.0  7.8  10.8  -  -
6.8  7.5  10.5  -  -
Average  6.5  7.6  10.3  -  -
SD  0.4  0.1  0.5  -  -
Positive  3/3  3/3  3/3  0/3  0/3
RT-qPCR  NIID  Ct value  30.93  34.08  37.59  39.08  -
30.79  34.12  38.05  39.34  -
30.60  33.79  37.61  -  -
Average  30.77  34.00  37.75  39.21  -
SD  0.14  0.15  0.21  0.13  -
copies/ reaction  2036.9  214.4  17.5  6.1  -
2249.6  208.6  12.7  5.0  -
2577.0  264.4  17.3  -  -
Average  2287.8  229.1  15.9  5.6  -
SD  222.1  25.0  2.2  0.5  -
Positive  3/3  3/3  3/3  2/3  0/3
WHO  Ct value  29.31  32.80  35.96  38.93  -
29.23  32.72  35.94  -  -
29.41  32.79  35.55  -  -
Average  29.32  32.77  35.81  38.93  -
SD  0.07  0.03  0.19  0.00  -
copies/ reaction  3503.2  254.9  23.7  2.6  -
3694.3  269.0  24.1  -  -
3246.6  256.5  32.4  -  -
Average  3481.4  260.2  26.7  2.6  -
SD  183.5  6.3  4.0  0.0  -
Positive  3/3  3/3  3/3  1/3  0/3