Amino acid sequences of Chiroptera ACE2 living in Italy were retrieved from UniProt database. The sequences of Rhinolophus ferrumequinum (UniProt ID: E2DHI2 and B6ZGN7) and Myotis daubentonii (UniProt ID: E2DHI8), were aligned to hACE2 sequence (UniProt ID: Q9BYF1) using Clustal Omega [35, 36]. Residues 1–358, including all the amino acids interacting with S protein RBD, were considered for alignment.