C for the specified time points until different assays had been performed. Viral propagation in IPEC-J2 cells was evaluated by observing cytopathic effect (CPE), determination of one-step growth curve and immunofluorescence assay (IFA) using the mAb 1A3 against PDCoV.

2.3  TCID50 Assay
The kinetics of PDCoV multiplication in IPEC-J2 cells was determined by measuring the TCID50 u