yl (C); maximum missed cleavages, 2; precursor mass tolerance, 10 ppm; fragment mass tolerance, 0.05 Da; validation based on, q-value. To guarantee the accuracy of the MS data analysis, the cutoff value for the peptide and protein confidences was set to >95% and >1.20, respectively, coupled with a false discovery rate (FDR) of ≤1% for peptide and protein identifications.