T2 measurements of ligands are also useful for determining the binding nature of a small molecule. The T2 values of small molecules are quite large compared to those of bigger molecules (i.e., proteins) mostly because macromolecules have more spin-spin diffusion [175]. Bound ligands will, therefore, display shorter T2 values than non-binding ligands because they interact with the target (i.e., protein), adopting similar vibrational and rotational energies to the target [176]. This interaction is represented by the resonance line broadening in the binding ligand’s spectrum when a receptor is introduced into the sample [156]. Given the sizable difference of T2 values of binding and non-binding ligands, one can utilize 1D relaxation-edited experiments to distinguish the binding ligands from the non-binding ligands efficiently and effectively based on the differences in the T2 values [167]. These and other related relaxation edited experiments prove useful in drug design.