Fortunately, significant efforts have been undertaken to reduce the amount of time it takes to record multidimensional spectra, especially for 2D NMR, and still obtain high quality spectra (Table 3). Novel pulse sequences have been developed to decouple nuclei in Pure-shift NMR [117]. Dynamic Nuclear Polarization (DNP) can induce hyper-polarization in atoms (13C, 15N) with an inherently low sensitivity [453,454]. Parahydrogen-Induced Polarization (PHIP) and Signal Amplification by Reversible Exchange (SABRE) are other polarization techniques used to increase the sensitivity of inherently insensitive nuclei [455].