Another method, called ultrafast 2D NMR, enables obtaining a 2D spectra within a single scan but with the associated cost of reduced sensitivity [117,448]. The principle of this method is to divide the sample into n number of fractions, and apply the appropriate incremental aspect to each fraction, while recording them all simultaneously within the one scan [448]. This method has been applied to many metabolomic studies [177,447,449] as well as in the analysis of natural products [450]. The difficulty is that the effective concentration of the sample is lowered by the fractionation level. The more slices the sample is split into, the greater the reduction of combined signal that is obtained.