| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T357 |
0-141 |
Sentence |
denotes |
Assays were assembled in total reaction volumes of either 12.5 μl (for LAMP assays using isolated RNA) or 20 μl (for swab–to–RT-LAMP assays). |
| T358 |
142-476 |
Sentence |
denotes |
Master mixes were prepared at room temperature for each reaction immediately before use with either 6.25 or 10 μl, respectively, of the WarmStart Colorimetric RT-LAMP 2X Master Mix (M1800, New England Biolabs) and 1.25 or 2 μl, respectively, of the 10× primer mix, filled up to 11.5 or 19 μl with nuclease-free water (AM9937, Ambion). |
| T359 |
477-511 |
Sentence |
denotes |
Values given are for one reaction: |
| T360 |
512-791 |
Sentence |
denotes |
For a 96-well plate, 100 times larger volumes were used, and the LAMP mix was distributed to the wells of a 96-well plate (4ti-0960/C, Brooks Life Sciences or 0030128672, Eppendorf) before pipetting 1 μl of sample into each well of the plate; for details, see previous paragraph. |
| T361 |
792-973 |
Sentence |
denotes |
Plates were prepared immediately before use to limit exposure of the LAMP reagents to atmospheric CO2 (to prevent acidification of the reaction) and kept on an ice-cold metal block. |
| T362 |
974-1157 |
Sentence |
denotes |
Plates were sealed using a transparent adhesive foil (GK480-OS, Kisker Biotech), and the reactions were incubated in a PCR cycler at 65°C for 15 to 60 min with the lid heated to 75°C. |
| T363 |
1158-1308 |
Sentence |
denotes |
To perform measurements at the indicated time points, the reactions were taken out of the PCR cycler and placed into an ice cold metal block for 30 s. |
| T364 |
1309-1359 |
Sentence |
denotes |
This intensifies the color before the measurement. |
| T365 |
1360-1460 |
Sentence |
denotes |
Photographs were taken with cell phone cameras or the scanner function of an office copying machine. |
