PMC:7574920 / 38152-39226 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7574920","sourcedb":"PMC","sourceid":"7574920","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7574920","text":"With its good sensitivity for samples up to CT ≈ 30, the colorimetric RT-LAMP assay has several advantages: It is fast, inexpensive, and it can be evaluated without any equipment. RT-LAMP reactions also appear to be less sensitive to contaminants in the samples than RT-qPCR, but care has to be taken that the samples used do not alter the pH as the colorimetric RT-LAMP assay is performed under conditions of weak pH buffering. Some clinical samples contain contaminants that can lead to acidification of the reaction independent of the presence of a template RNA if too much sample is added. Diagnostic RT-qPCR tests usually include a technical internal control, i.e., another RNA species, which is spiked into all samples and which is detected independent of the gene of interest to safeguard against the possibility of a general reaction failure within a sample tube. It would be desirable to have a similar precaution for the RT-LAMP assay. A multiplexed fluorescence readout might provide this (34) but comes at the expense of the simplicity of a colorimetric 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