RT-LAMP CT Pos Neg Sum LAMP- sequencing Pos RT-qPCR Pos 0–25 49 0 49 25–30 28 0 28 30–35 4 0 4 35–40 0 0 0 Neg Neg 0 0 0 Neg RT-qPCR Pos 0–25 0 0 0 25–30 0 2 2 30–35 0 16 16 35–40 0 16 16 Neg Neg 2 637 639 Too few reads RT-qPCR Pos 0–25 2 0 2 25–30 0 0 0 30–35 0 0 0 35–40 0 0 0 Neg Neg 0 12 12 Sum 85 683 768