To determine the specificity and sensitivity of the RT-LAMP assay, we continued to analyze more RNA samples. We assayed a total of 768 RNA samples obtained on different days (fig. S1). Visualization of the RT-LAMP assay results 30 min after the start of the incubation at 65°C showed comparable behavior of the samples in a total of ten 96-well test plates (Fig. 3A and Table 1), indicating that the RT-LAMP assay was reproducible from day to day and from plate to plate.