The fifth protocol of the Registered Report, which was to test if knockdown of ceRNAs resulted in AKT activation in DU145, wild-type HCT116, and DicerEx5 HCT116 cells was conducted, but the results obtained were inconclusive. The Western blot results with the phospho-AKT antibody were inconsistent and largely absent of a signal. This was not due to the Western blot technique as other antibodies gave reliable singles, as illustrated in Figure 1B. Instead, the result is likely due to the lack of phosphatase inhibitors included in the lysis buffer. Of note, the original study also did not use phosphatase inhibitors. Since the primary outcome was not able to be observed, the experiment was not included in this manuscript. Results are available at https://osf.io/ju597/. Additional materials and instrumentation not listed in the Registered Report, but needed during experimentation are also listed above.