PMC:7571312 / 126081-126238 JSONTXT

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T161","span":{"begin":54,"end":55},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T162","span":{"begin":139,"end":143},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"}],"text":" resuspended and lysed by microfluidization in Buffer A (20 mM Tris pH 8.0 + 150 mM NaCl). Lysates were clarified at 25,000 × g for 1 h at 4 °C. The soluble "}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T2872","span":{"begin":63,"end":67},"obj":"Chemical"},{"id":"T39293","span":{"begin":84,"end":88},"obj":"Chemical"}],"attributes":[{"id":"A85879","pred":"chebi_id","subj":"T2872","obj":"http://purl.obolibrary.org/obo/CHEBI_9754"},{"id":"A30734","pred":"chebi_id","subj":"T39293","obj":"http://purl.obolibrary.org/obo/CHEBI_26710"}],"text":" resuspended and lysed by microfluidization in Buffer A (20 mM Tris pH 8.0 + 150 mM NaCl). Lysates were clarified at 25,000 × g for 1 h at 4 °C. The soluble "}

{"project":"LitCovid-sentences","denotations":[{"id":"T684","span":{"begin":91,"end":144},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":" resuspended and lysed by microfluidization in Buffer A (20 mM Tris pH 8.0 + 150 mM NaCl). Lysates were clarified at 25,000 × g for 1 h at 4 °C. The soluble "}

{"project":"LitCovid-PubTator","denotations":[{"id":"2205","span":{"begin":63,"end":67},"obj":"Chemical"},{"id":"2206","span":{"begin":84,"end":88},"obj":"Chemical"}],"attributes":[{"id":"A2206","pred":"tao:has_database_id","subj":"2206","obj":"MESH:D012965"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":" resuspended and lysed by microfluidization in Buffer A (20 mM Tris pH 8.0 + 150 mM NaCl). Lysates were clarified at 25,000 × g for 1 h at 4 °C. The soluble "}