PMC:7571312 / 122500-124139
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7571312","sourcedb":"PMC","sourceid":"7571312","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7571312","text":"All assays were performed in BSL3 containment. Vero 76 cells were plated at 10 000 cells per well using phenol red-free DMEM or IMEM (Gibco). After adhering for 2 h at 37 °C, various concentrations of compound (320, 100, 33, 10, 3.3, 1.0, 0.3, or 0.1 μM) were added and the cells were infected with 2.6 × 103 PFU/well SARS CoV Toronto-2 (provided as a gift from Dr. Heinz Feldman (NIAID, Hamilton, MT)) or mock-infected with the medium only. After 66 h, cell viability was determined using either the neutral red method or the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI). For the neutral red method, the cells were washed twice with PBS, and 100 μL of DMEM pH 4.5 containing 0.066% neutral red was added to the cells for 2 h at 37 °C. The cells were again washed twice with PBS, 100 μL of buffer solution (50% EtOH, 1% acetic acid) was added, and the OD was read at 540 nM after a 10 min incubation at 37 °C with shaking. Data are expressed as the percent of neutral red or luminescent signal in wells of compound-treated cells compared to the signal in wells of uninfected, compound-free cells. The 50% effective concentration (EC50) is calculated as the concentration of the compound that increases the percent of the neutral red or luminescent signal in infected, compound-treated cells to 50% of that produced by uninfected, compound-free cells. The 50% cytotoxicity concentration (CC50) is calculated as the concentration of the compound that decreases the percent of the neutral red or luminescent signal in uninfected, compound-treated cells to 50% of that produced in uninfected, compound-free cells.51,52","tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T7","span":{"begin":55,"end":60},"obj":"Body_part"},{"id":"T8","span":{"begin":83,"end":88},"obj":"Body_part"},{"id":"T9","span":{"begin":274,"end":279},"obj":"Body_part"},{"id":"T10","span":{"begin":454,"end":458},"obj":"Body_part"},{"id":"T11","span":{"begin":553,"end":557},"obj":"Body_part"},{"id":"T12","span":{"begin":630,"end":635},"obj":"Body_part"},{"id":"T13","span":{"begin":737,"end":742},"obj":"Body_part"},{"id":"T14","span":{"begin":765,"end":770},"obj":"Body_part"},{"id":"T15","span":{"begin":1048,"end":1053},"obj":"Body_part"},{"id":"T16","span":{"begin":1115,"end":1120},"obj":"Body_part"},{"id":"T17","span":{"begin":1310,"end":1315},"obj":"Body_part"},{"id":"T18","span":{"begin":1369,"end":1374},"obj":"Body_part"},{"id":"T19","span":{"begin":1569,"end":1574},"obj":"Body_part"},{"id":"T20","span":{"begin":1628,"end":1633},"obj":"Body_part"}],"attributes":[{"id":"A7","pred":"fma_id","subj":"T7","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A8","pred":"fma_id","subj":"T8","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A9","pred":"fma_id","subj":"T9","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A10","pred":"fma_id","subj":"T10","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A11","pred":"fma_id","subj":"T11","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A12","pred":"fma_id","subj":"T12","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A13","pred":"fma_id","subj":"T13","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A14","pred":"fma_id","subj":"T14","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A15","pred":"fma_id","subj":"T15","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A16","pred":"fma_id","subj":"T16","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A17","pred":"fma_id","subj":"T17","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A18","pred":"fma_id","subj":"T18","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A19","pred":"fma_id","subj":"T19","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A20","pred":"fma_id","subj":"T20","obj":"http://purl.org/sig/ont/fma/fma68646"},{"subj":"T7","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T8","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T9","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T10","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T11","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T12","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T13","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T14","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T15","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T16","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T17","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T18","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T19","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T20","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T105","span":{"begin":318,"end":322},"obj":"Disease"},{"id":"T106","span":{"begin":877,"end":879},"obj":"Disease"}],"attributes":[{"id":"A105","pred":"mondo_id","subj":"T105","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A106","pred":"mondo_id","subj":"T106","obj":"http://purl.obolibrary.org/obo/MONDO_0017178"},{"subj":"T105","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T106","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T116","span":{"begin":47,"end":54},"obj":"http://purl.obolibrary.org/obo/CLO_0009526"},{"id":"T117","span":{"begin":55,"end":60},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T118","span":{"begin":83,"end":88},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T119","span":{"begin":274,"end":279},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T120","span":{"begin":350,"end":351},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T121","span":{"begin":454,"end":458},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T122","span":{"begin":553,"end":557},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T123","span":{"begin":630,"end":635},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T124","span":{"begin":737,"end":742},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T125","span":{"begin":765,"end":770},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T126","span":{"begin":905,"end":909},"obj":"http://purl.obolibrary.org/obo/CLO_0001550"},{"id":"T127","span":{"begin":1012,"end":1018},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T128","span":{"begin":1048,"end":1053},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T129","span":{"begin":1070,"end":1076},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T130","span":{"begin":1115,"end":1120},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T131","span":{"begin":1273,"end":1279},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T132","span":{"begin":1310,"end":1315},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T133","span":{"begin":1369,"end":1374},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T134","span":{"begin":1530,"end":1536},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T135","span":{"begin":1569,"end":1574},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T136","span":{"begin":1628,"end":1633},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"attributes":[{"subj":"T116","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T117","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T118","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T119","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T120","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T121","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T122","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T123","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T124","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T125","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T126","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T127","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T128","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T129","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T130","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T131","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T132","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T133","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T134","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T135","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T136","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T9229","span":{"begin":104,"end":114},"obj":"Chemical"},{"id":"T16403","span":{"begin":104,"end":110},"obj":"Chemical"},{"id":"T61020","span":{"begin":398,"end":400},"obj":"Chemical"},{"id":"T87606","span":{"begin":501,"end":512},"obj":"Chemical"},{"id":"T53163","span":{"begin":593,"end":595},"obj":"Chemical"},{"id":"T16600","span":{"begin":606,"end":617},"obj":"Chemical"},{"id":"T42830","span":{"begin":708,"end":719},"obj":"Chemical"},{"id":"T67130","span":{"begin":815,"end":821},"obj":"Chemical"},{"id":"T31728","span":{"begin":822,"end":830},"obj":"Chemical"},{"id":"T31154","span":{"begin":836,"end":840},"obj":"Chemical"},{"id":"T66158","span":{"begin":845,"end":856},"obj":"Chemical"},{"id":"T49097","span":{"begin":852,"end":856},"obj":"Chemical"},{"id":"T82790","span":{"begin":985,"end":996},"obj":"Chemical"},{"id":"T16617","span":{"begin":1246,"end":1257},"obj":"Chemical"},{"id":"T95918","span":{"begin":1503,"end":1514},"obj":"Chemical"}],"attributes":[{"id":"A81740","pred":"chebi_id","subj":"T9229","obj":"http://purl.obolibrary.org/obo/CHEBI_31991"},{"id":"A97066","pred":"chebi_id","subj":"T16403","obj":"http://purl.obolibrary.org/obo/CHEBI_15882"},{"id":"A24884","pred":"chebi_id","subj":"T61020","obj":"http://purl.obolibrary.org/obo/CHEBI_73614"},{"id":"A76753","pred":"chebi_id","subj":"T87606","obj":"http://purl.obolibrary.org/obo/CHEBI_86370"},{"id":"A39457","pred":"chebi_id","subj":"T53163","obj":"http://purl.obolibrary.org/obo/CHEBI_141448"},{"id":"A31729","pred":"chebi_id","subj":"T16600","obj":"http://purl.obolibrary.org/obo/CHEBI_86370"},{"id":"A73029","pred":"chebi_id","subj":"T42830","obj":"http://purl.obolibrary.org/obo/CHEBI_86370"},{"id":"A74832","pred":"chebi_id","subj":"T67130","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A68565","pred":"chebi_id","subj":"T31728","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A83044","pred":"chebi_id","subj":"T31154","obj":"http://purl.obolibrary.org/obo/CHEBI_16236"},{"id":"A93061","pred":"chebi_id","subj":"T66158","obj":"http://purl.obolibrary.org/obo/CHEBI_15366"},{"id":"A27039","pred":"chebi_id","subj":"T49097","obj":"http://purl.obolibrary.org/obo/CHEBI_37527"},{"id":"A94316","pred":"chebi_id","subj":"T82790","obj":"http://purl.obolibrary.org/obo/CHEBI_86370"},{"id":"A54471","pred":"chebi_id","subj":"T16617","obj":"http://purl.obolibrary.org/obo/CHEBI_86370"},{"id":"A47269","pred":"chebi_id","subj":"T95918","obj":"http://purl.obolibrary.org/obo/CHEBI_86370"},{"subj":"T9229","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T16403","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T61020","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T87606","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T53163","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T16600","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T42830","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T67130","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T31728","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T31154","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T66158","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T49097","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T82790","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T16617","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T95918","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T661","span":{"begin":0,"end":46},"obj":"Sentence"},{"id":"T662","span":{"begin":47,"end":141},"obj":"Sentence"},{"id":"T663","span":{"begin":142,"end":441},"obj":"Sentence"},{"id":"T664","span":{"begin":442,"end":597},"obj":"Sentence"},{"id":"T665","span":{"begin":598,"end":760},"obj":"Sentence"},{"id":"T666","span":{"begin":761,"end":947},"obj":"Sentence"},{"id":"T667","span":{"begin":948,"end":1121},"obj":"Sentence"},{"id":"T668","span":{"begin":1122,"end":1375},"obj":"Sentence"},{"id":"T669","span":{"begin":1376,"end":1639},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T661","pred":"source","obj":"LitCovid-sentences"},{"subj":"T662","pred":"source","obj":"LitCovid-sentences"},{"subj":"T663","pred":"source","obj":"LitCovid-sentences"},{"subj":"T664","pred":"source","obj":"LitCovid-sentences"},{"subj":"T665","pred":"source","obj":"LitCovid-sentences"},{"subj":"T666","pred":"source","obj":"LitCovid-sentences"},{"subj":"T667","pred":"source","obj":"LitCovid-sentences"},{"subj":"T668","pred":"source","obj":"LitCovid-sentences"},{"subj":"T669","pred":"source","obj":"LitCovid-sentences"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"2142","span":{"begin":318,"end":326},"obj":"Species"},{"id":"2143","span":{"begin":104,"end":114},"obj":"Chemical"},{"id":"2144","span":{"begin":120,"end":124},"obj":"Chemical"},{"id":"2145","span":{"begin":659,"end":662},"obj":"Chemical"},{"id":"2146","span":{"begin":678,"end":682},"obj":"Chemical"},{"id":"2147","span":{"begin":800,"end":803},"obj":"Chemical"},{"id":"2148","span":{"begin":836,"end":840},"obj":"Chemical"},{"id":"2149","span":{"begin":845,"end":856},"obj":"Chemical"},{"id":"2150","span":{"begin":285,"end":293},"obj":"Disease"},{"id":"2151","span":{"begin":411,"end":419},"obj":"Disease"},{"id":"2152","span":{"begin":1283,"end":1291},"obj":"Disease"},{"id":"2153","span":{"begin":1384,"end":1396},"obj":"Disease"}],"attributes":[{"id":"A2142","pred":"tao:has_database_id","subj":"2142","obj":"Tax:694009"},{"id":"A2143","pred":"tao:has_database_id","subj":"2143","obj":"MESH:D010637"},{"id":"A2145","pred":"tao:has_database_id","subj":"2145","obj":"MESH:D007854"},{"id":"A2147","pred":"tao:has_database_id","subj":"2147","obj":"MESH:D007854"},{"id":"A2148","pred":"tao:has_database_id","subj":"2148","obj":"MESH:D000431"},{"id":"A2149","pred":"tao:has_database_id","subj":"2149","obj":"MESH:D019342"},{"id":"A2150","pred":"tao:has_database_id","subj":"2150","obj":"MESH:D007239"},{"id":"A2151","pred":"tao:has_database_id","subj":"2151","obj":"MESH:D007239"},{"id":"A2152","pred":"tao:has_database_id","subj":"2152","obj":"MESH:D007239"},{"id":"A2153","pred":"tao:has_database_id","subj":"2153","obj":"MESH:D064420"},{"subj":"2142","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2143","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2144","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2145","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2146","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2147","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2148","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2149","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2150","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2151","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2152","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2153","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"2_test","denotations":[{"id":"33054210-3983963-61913462","span":{"begin":1634,"end":1636},"obj":"3983963"},{"id":"33054210-28359770-61913463","span":{"begin":1637,"end":1639},"obj":"28359770"}],"attributes":[{"subj":"33054210-3983963-61913462","pred":"source","obj":"2_test"},{"subj":"33054210-28359770-61913463","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#93c0ec","default":true},{"id":"LitCovid-PD-MONDO","color":"#dbec93"},{"id":"LitCovid-PD-CLO","color":"#e393ec"},{"id":"LitCovid-PD-CHEBI","color":"#93ecc9"},{"id":"LitCovid-sentences","color":"#ecaf93"},{"id":"LitCovid-PubTator","color":"#9593ec"},{"id":"2_test","color":"#aaec93"}]}]}}