Table 1  Diagnostic criteria of the AspICU clinical algorithm according to Blot et al., 2012, and proposal of a modified AspICU algorithm.
Classification  AspICU According to Blot et al., 2012 [3]  AspICU Algorithm Incorporating PCR  Modified AspICU Algorithm Incorporating PCR, Serology and Angioinvasion Biomarkers
Definition of colonization  Aspergillus-positive culture endotracheal aspirate alone  Aspergillus-positive culture/PCR endotracheal aspirate alone  Aspergillus-positive culture/PCR endotracheal aspirate in one sample, not confirmed on a second sample or using blood biomarker
Definition of putative IA  >1 criterion among: 1. Aspergillus-positive culture endotracheal aspirate 2. Compatible clinical signs 3. Abnormal thoracic medical imaging on CT scan or X-ray 4a. Host risk factors Or 4b. Semiquantitative Aspergillus-positive culture of BAL fluid + positive direct microscopy  >1 criterion among: 1. Aspergillus-positive culture/PCR endotracheal aspirate 2. Compatible clinical signs 3. Abnormal thoracic medical imaging on CT scan or X-ray 4a. Host risk factors Or 4b. Semiquantitative Aspergillus-positive culture/PCR of BAL fluid + positive direct microscopy  >1 criterion among: 1. Aspergillus-positive culture/PCR endotracheal aspirate in repeated samples with negative anti-Aspergillus antibody testing 2. Compatible clinical signs 3. Abnormal thoracic medical imaging on CT scan or X-ray 4a. Host risk factors Or 4b. Semiquantitative Aspergillus-positive culture/PCR of BAL fluid + positive direct microscopy
Definition of probable IA  -  -  Putative IA + one positive blood biomarker (GM and/or PCR)
Definition of proven IA  Positive histopathology  Positive histopathology  Positive histopathology
GM: galactomannan.