Estimation of Nitrate-Nitrogen (NO 3-N) Concentration Value for Optimisation of Bioremediation Cocktail Model Design
Nitrate- nitrogen concentration in corn steep liquor = 3.31mgL -1 = 0.00331mgml -1
Bacterial-substrate growth and variance analysis of the OVAT experimental data for corn steep liquor estimated optimal nitrate substrate to range from 10 to 20ml of the corn steep liquor for optimal bacterial specific growth rate and enzyme activity. The experiment was set up in 100ml of growth media.
Scaling up the laboratory data to bioremediation setup, mean multiplying by a factor of 10 (100ml to 1000ml). Where 1000ml of water = 1kg = 1000g (2000g of soil used in remediation setup)
Therefore, corn steep liquor for bioremediation ranged from 100ml to 200ml.
Resulting in a Nitrate-Nitrogen (NO3-N) concentration of 0.331mg/100ml to 0.662mg/200ml for a kilogram of the sample soil treated in the bioremediation setup.