Cell viability assay was carried out in Raw264.7 cells using MTT dye (3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide) as reported earlier (Dogra et al., 2019). Cells were seeded in a 96-well plate and allowed to grow overnight. Further cells were treated with varying doses (0, 2, 5, 10, 15, 20, 30, and 40 µM) of the LDN for 24 h. Post-treatment, 10 µl of MTT (5 mg/ml stock in PBS) was added to all the wells. The formazan crystals thus formed were solubilized in 200 µl DMSO and the absorbance was recorded using (Infinite M200 Pro TECAN).