Several live imaging modalities have been developed and optimized for detection of proteinaceous aggregates and metabolic hyperspectral mapping of retinal tissue in rodent models of AD (Koronyo-Hamaoui et al., 2011; Koronyo et al., 2012, 2017; Schön et al., 2012; More and Vince, 2015; Sidiqi et al., 2020). Using longitudinal scanning laser ophthalmology, Schön et al. visualized and monitored pTau-containing RGCs in the P301S mouse model of tauopathy from 2 to 6.5 months of age and found a steady growth in pTau-positive cell counts (Schön et al., 2012).