Evidence of hyperphosphorylated pTau inclusions in AD retina. (A–E) Representative micrograph images of retinal cross-sections from AD patients following (A,B) immunostaining with AT8 mAb against phospho-tau (Ser202, Thr205), revealing intracellular pTau aggregates in both inner and outer nuclear layers (INL and ONL), and plexiform layers (IPL and OPL). Scale bars: 10 μm; (C,D) Higher magnification images of inner retina stained with Gallyas silver showing neurofibrillary tangle (NFT)-like structures in ganglion cell layer (GCL). (E) Immuofluorescence staining of AT100 mAb against phospho-tau (Thr212, Ser214) showing punctate aggregates and intracellular inclusions in INL. Images and data of panels (A,B) are adapted from Schön et al. (2012). Images and data of panels c-d are reproduced from Koronyo et al. (2017) with permission of ASCI via Copyright Clearance Center.