Overview of reconstruction volumes, exemplified for a punch biopsy of pulmonary autopsy sample I. (a) Virtual section through the stitched reconstruction volume with indicated position for the 1 mm biopsy punch. (b) Virtual section through the reconstruction of the 1 mm biopsy punch volume with indicated position for the cone-beam dataset. (c) Virtual section through the cone beam dataset. (d) Image of an HE-stained histological section, which is in close proximity to the virtual slice in (a). (e) 3D views of the biopsy punch. (f) 3D views of the cone-beam datasets. Scale bars: (a) 500⁢μ⁢m and (b,c) 100⁢μ⁢m. The entire reconstruction volume is visualized in Video 1.