Figure 4  Viral HBV L, but not subviral HBV S, colocalizes with ERGIC-53 upon ER export block. (A) Domain structures of the S and L proteins (left), their transmembrane topology at the ER (middle) and HBV subviral particle (SVP) and VP types (right). The red circles depict the N-glycan acceptor N146. (B,C) Cells were treated with the indicated RNA duplexes for 2 days and retransfected with pHBV.S (B) or pHBV* (C). After an additional two days, cells were fixed with PFA and stained with either rabbit anti-S (K38, S, green) (B) or rabbit anti-L (57761, L, green) (C) plus mouse anti-ERGIC-53 (red) antibodies. DNA staining is shown in blue. Overlaid images (yellow) are displayed in the Merge panels with magnifications and PCC data. Bar, 10 µM.