Experimental Model and Subject Details
HEK293-F cells (GIBCO) were maintained and passaged in FreeStyle Media (GIBCO) containing 1% Pen Strep (GIBCO). Vero-6 cells (ATCC) were maintained and passaged in DMEM medium supplemented with 10% fetal bovine serum and 1% Pen Strep (GIBCO) and amphotericin B antibiotics. All cells were maintained at 37°C with 5% CO2 before and after transfection.