together, these data can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.

Graphical Abstract

Highlights
•  Site-specific N-linked microheterogeneity is defined at 22 sites of SARS-CoV-2 Spike
•  Six sites of N-linked microheterogeneity of human ACE2 receptor are described
•  Molecular dynamics simulations of Spike and ACE2 show essential roles for glycosylation
•  We uncover roles for variants in protein-glycan and glycan-glycan interactions

Combining g