Cotton fabric, which was ready for use by the textile industry, was LbL nanocoated. The alternating layers comprised of PSS and poly(allylamine) bilayers followed by bilayers of PSS and chitosan. The coated fabric was washed in water to remove the excess polyelectrolyte with or without ultrasonication. The fabric that was washed with ultrasonication produced a more uniformly coated fabric than without. The coated material was exposed to antimicrobial tests and significant antibacterial activity was shown toward S. aureus and some activity was still maintained after washing the fabric with a non-ionic detergent. Up to 80% of bactericidal activity was maintained for fabric samples that were washed with ultrasonication compared to samples washed without. The latter samples retained approximately 30% bactericidal effect. Antiviral effects were not investigated [163].