Figure 6  Fatty acid profile of experimental diets and liver; and hepatic lipid content, lipogenic gene expression and inflammatory markers in mice. (A) Myristic, (B) Capric, (C) Stearic, (D) Arachidonic, (E) Eicosapentaenoic and (F) Conjugated linoleic fatty acids content in diets and liver. (G) Hematoxylin & eosin and oil red O (ORO) staining in liver sections, (H) ORO densitometric quantitation, (I) SREBP-1c mRNA abundance. (J) Correlation between SREBP-1c mRNA in liver and DHA content in liver. (K) Phospho-AMPK, total AMPK, phospho-JNK and total JNK immunoblots. (L) Densitometric analysis of phospho-AMPK/AMPK ratio and (M) Phospho-JNK/JNK ratio. (N) Tumor necrosis factor α (TNF-α) content in liver, and (O) EPA+DHA/AA ratio in liver of mice fed control diet (control), a high-fat diet (HF) or a high-fat diet with milk from goats fed a conventional diet (HFCD), from goats grazing (HFG) or from goats fed a CD supplemented with Acacia farnesiana pods (HFAF). Results are presented as the mean ± S.E.M., n = 6 mice per group and analyzed by one-way ANOVA followed by Tukey multiple comparison post hoc test. The differences were considered statistically significant at p < 0.05. Mean values with different lowercase letters show statistical differences between each other.