Figure 4  Histological and molecular features of brown adipose tissue and skeletal muscle (A) Adipocyte size BAT, (B) Uncoupling protein one (UCP-1) immunohistochemistry of BAT, (C) UCP-1 immunoblot, (D) Densitometric analysis of UCP-1, (E) Lipid content (BODIPY staining) and mitochondrial activity (SDH staining) in skeletal muscle, (F) Densitometric quantification of BODIPY staining, (G) Densitometric quantification of SDH staining, (H) Total AMPK and phospho-AMPK immunoblot, (I) p-AMPK/total AMPK densitometric analysis, (J) AMPK (K,L) PPARδ mRNA abundance in skeletal muscle of mice fed control diet (control), a high-fat diet (HF) or a high-fat diet with milk from goats fed a conventional diet (HFCD), from goats grazing (HFG) or from goats fed a CD supplemented with Acacia farnesiana pods (HFAF). Results are presented as the mean ± S.E.M., n = 6 mice per group and analyzed by one-way ANOVA followed by Tukey multiple comparison post hoc test. The differences were considered statistically significant at p < 0.05. Mean values with different lowercase letters show statistical differences between each other.