Data is expressed as mean ± standard error of the means (S.E.M.). Shaphiro-Wilk normality test was used to check data distribution. All groups were analyzed by one-way ANOVA followed by Tukey multiple comparison post hoc test using GraphPad Prism 7.0 (GraphPad Software, San Diego, CA, USA). The differences were considered statistically significant at p < 0.05. Mean values with different lowercase letters show statistical differences between each other.