4.4. Serum Determinations
Serum glucose, cholesterol and triacylglycerols (Tg) were determined using a unicel DxC 600 analyzer (Beckman Coulter, Brea, CA, USA). Insulin (cat. 80-INSMS-EO1, ALPCO, Salem, NH, USA) and leptin (cat. KMC228, Thermo Fisher Scientific, Waltham, MA, USA) were determined by ELISA assays according with the manufacturer’s recommendations.