At the end of the study, mice were deprived of food six hours previous to the euthanasia mice were deprived of food. Euthanasia was performed by exposing mice to a lethal dose of sevoflurane (fluoromethyl-2,2,2-trifluoro-1-(trifluoromethyl)ethyl ether). Total blood volume was drawn from the posterior vena cava using a 1 mL syringe coated with heparin. Serum was separated by centrifugation for 10 min at 1800× g at 4 °C. Then subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), brown adipose tissue (BAT), liver, pancreas, skeletal muscle (soleus and gastrocnemius) were rapidly removed and divided in smalls parts. Some samples were frozen in liquid nitrogen and stored at −80 °C. The rest of the samples were fixed in ice-cold 4% (w/v) paraformaldehyde in phosphate buffer saline (PBS) for histological analyses as described below.