Sequencing and analysis of TCR and BCR V(D)J
PCR amplification was done to enrich the full-length TCR/BCR V(D)J segments for the amplified cDNA from 5′ libraries with a Chromium Single-Cell V(D)J Enrichment kit (10 Genomics). The TCR/BCR sequences of each T/B cell were clustered using the CellRanger vdj pipeline (version 3.1.0, allowing identification of CDR3 sequence and the rearranged TCR/BCR gene. Analysis was performed using Loupe V(D)J Browser version 2.0.1 (https://support.10xgenomics.com, 10x Genomics). In summary, barcode information a containing clonotype frequency and TCR/BCR diversity metric were obtained. We projected T /B cells with dominant TCR/BCR clonotypes on a t-SNE plot using barcode information (Wen et al., 2020).