Figure 6 Poor outcomes upon COVID-19 infection is associated with imbalanced cellular aging. (A) t-SNE projections of PBMCs derived from mass cytometry data in cohort-2. (B) Heatmap showing mean population expression levels of all markers. (C) t-SNE plots segregated by HC and CO groups. HC includes YH (n = 2) and AH (n = 2); CO includes YCO (n = 2) and ACO (n = 2). (D) Percentage of immune cell populations in PBMC between HC (n = 4) and CO (n = 4) groups. (E) Bar chart of the relative percentage of major immune cell populations derived from mass cytometry data between HC and CO groups. (F) Percentage of CD4 Naive cells in CD45+ cells between HC (n = 4) and CO (n = 4) groups. (G) Percentage of NK2 cells in CD45+ cells between HC (n = 4) and CO (n = 4) groups. (H) Percentage of CD16 monocytes in CD45+ cells between HC (n = 4) and CO (n = 4) groups. (I) Bar chart of the relative percentage of major immune cells derived from mass cytometry data from YH, AH and ACO groups. (J) Bar chart of the relative percentage of T cell subsets derived from mass cytometry data from YH, AH and ACO groups. (K) Bar chart of the relative percentage of NK cell subsets derived from mass cytometry data from YH, AH and ACO groups. (L) Bar chart of the relative percentage of B cell subsets derived from mass cytometry data from YH, AH and ACO groups. (M) Bar chart of the relative percentage of DC subsets derived from mass cytometry data from YH, AH and ACO groups. (N) Bar chart of the relative percentage of monocyte subsets derived from mass cytometry data from YH, AH and ACO groups. (O) Bar chart of the relative percentage of major immune cell populations derived from mass cytometry data between YCO and ACO groups. (P) CT photography showing the different evolution of Lung Ground-Glass Opacity in young and aged patients with COVID-19. CT, computed tomography. P values are based on two-tailed Mann-Whitney-Wilcoxon tests between groups